The Biological Activities of 1α, 25-Dihydroxyvitamin D_3 and Its Synthetic Analog 1α, 25-Dihydroxy-16-ene-vitamin D_3 in Normal Human Osteoblastic Cells and Human Osteosarcoma SaOS-2 Cells Are Modulated by 17-β Estradiol and Dependent on Stage of Differen

スポンサーリンク

概要

• 論文の詳細を見る

• We compared the effects of 1α, 25-dihydroxyvitamin D_3 [1α, 25(OH)_2D_3]and its analog, 1α, 1α, 25-dihydroxy-16-ene-vitamin D_3[1α, 25(OH)_2-16-ene-D_3], as well as their interactions with 17-β estradiol(E_2)on osteoblastic function in our human normal(HOB)and osteosarcoma SaOS-2 cell models representing two different stages of differentiation, the more diffirentiated HOB+DEX cells and SaOS-2 cell models represebting two different stages of differentiation, the more differentiated HOB+DEX cells and SaOS+DEX cells, and the corresponding less differentiated HOB-DEX and SaOS-DEX cells. The differential effects of 1α, 25(OH)_2D_3 and 1α, 25(OH)_2-16-ene-D_3 and the modulation by E_2 on ALP activity in HOB-DEX and HOB+DEX cells were small but significant. The most signficant effects were seen in SaOS+DEX cells, in which 1α, 25(OH)_2-16-ene-D_3 was 100-fold more potent than 1α, 25(OH)_2D_3 the maximal enhancement being exerted at 0.1nм and 10nм, recepectively. E_2 enhanced the stimulatory effects of both compounds, with ALP being increased 2-fold at 0.1nм(p<0.001). Osteocalcin(OC)production in HOB-DEX cells was stimulated 1.3 to 1.4-fold by 1α, 25(OH)_2D_3 and 1α, 25(OH)_2-16-ene-D_3 at a concentration of 0.01nм, with E_2 inhibiting the effect of 1α, 25(OH)_2-16-ene-D_3. In SaOS-DEX and SaOS+DEX cells, 1α, 25(OH)_2D_3 and 1α, 25(OH)_2-16-ene-D_3 stimulated OC production 1.6-fold at 0.1nм with E_2 slightly enhancing the effect of 1α, 25(OH)_2D_3 was larger than that of 1α, 25(OH)_2-16-ene-D_3. These results shows that 1α, 25(OH)_2-16-ene-D_3 is biologically active in human osteoblasts.

• 公益社団法人日本薬学会の論文
• 2001-03-01

著者

• RAO Leticia

  Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and De

• LIU Lily

  Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and De

• MURRAY Timothy

  Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and De

• Liu Lily

  Calcium Research Laboratory St.michael's Hospital Department Of Medicine University Of Toronto

• Rao L

  Univ. Toronto Ontario Can

• Rao Leticia

  Calcium Research Laboratory Division Of Endocrinology And Metabolism St. Michael's Hospital And

• Uskokovic Milan

  Providence Ri U.s.a. And Hoffman-laroche

• Murray Timothy

  Calcium Research Laboratory St.michael's Hospital Department Of Medicine University Of Toronto

• RAWLINS Myrna

  Calcium Research Laboratory, St.Michael's Hospital, Department of Medicine, University of Toronto

• MCBROOM Robert

  Department of Orthopaedics & Surgery, St.Michael's Hospital, Department of Medicine, University of T

• REDDY G.Satyanarayama

  Department of Pediatrics Women and Infants'Hospital, Brown University School of Medicine

• RAO D.Sunita

  Department of Pediatrics Women and Infants'Hospital, Brown University School of Medicine

• SUTHERLAND May

  Calcium Research Laboratory, St.Michael's Hospital, Department of Medicine, University of Toronto

• Rao D.sunita

  Department Of Pediatrics Women And Infants'hospital Brown University School Of Medicine

• Rawlins Myrna

  Calcium Research Laboratory St.michael's Hospital Department Of Medicine University Of Toronto

• Mcbroom Robert

  Department Of Orthopaedics & Surgery St.michael's Hospital Department Of Medicine Universit

• Sutherland May

  Calcium Research Laboratory St.michael's Hospital Department Of Medicine University Of Toronto

• Reddy G.satyanarayama

  Department Of Pediatrics Women And Infants'hospital Brown University School Of Medicine

関連論文

• Estrogen Added Intermittently, but Not Continuously, Stimulates Differentiation and Bone Formation in SaOS-2 Cells
• The Biological Activities of 1α, 25-Dihydroxyvitamin D_3 and Its Synthetic Analog 1α, 25-Dihydroxy-16-ene-vitamin D_3 in Normal Human Osteoblastic Cells and Human Osteosarcoma SaOS-2 Cells Are Modulated by 17-β Estradiol and Dependent on Stage of Differen
• Calcium from LactoCalcium^ Milk Mineral after Digestion with Pepsin Stimulates Mineralized Bone Nodule Formation in Human Osteoblast-Like SaOS-2 Cells in Vitro and May Be Rendered Bioavailable in Vivo

スポンサーリンク