Identification of Intracellular Localization of Laminin in the Rat Anterior Pituitary(COMMUNICATION)(Endocrinology)

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概要

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• The intracellular localization of laminin of endocrine cells in the rat anterior pituitary was examined by biochemical methods and electron-microscopical immunocytochemistry. The secretory granules of the anterior pituitary cells were separated. And the granules were immunostained by the post-embedding method using antiserum against laminin. Laminin was detected on the surface of the membrane of separated granules. On the other hand, the granules were immunoblotted using antiserum and monoclonal antibody against laminin. The antiserum reactive with both A-chain and B-chains of the antigen (mouse EHS sarcoma laminin) cross-reacted with only the B-chain(s) of the secretory granules, but the monoclonal antibody showed no cross-reactivity. These results suggest that laminin in the anterior pituitary cells may exist as B-chain singly in the secretory granules and it may be different from mouse EHS sarcoma laminin in structure.

• 社団法人日本動物学会の論文
• 1992-04-15

著者

• Namiki Hideo

  Department Of Biology Faculty Of Education And Integrated Arts And Sciences Waseda University

• KIKUTA Toshiteru

  Department of Biology, School of Education, Waseda University

• Kikuta T

  Waseda Univ. Tokyo Jpn

• Kikuta Toshiteru

  Department Of Biology School Of Education Waseda University

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