The Effect of Culture Plate Ventilation Space on Cell Growth in vitro(Cell Biology)

概要

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Human fetal lung fibroblast cells (TIG-3) and HeLa cells were cultured in a multiplate either covered with a lid (lid^+) or, alternatively without a lid (lid^-). In case of TIG-3, proliferation of the cells in the lid^- plate was significantly less as compared with that in the lid^+ plate, regardless of the serum concentration. On the contrary, HeLa cells significantly increased in cell number in the lid^- plate. Two kinds of culture media, one supplemented with fetal bovine serum and the other supplemented with the freeze-dried fetal bovine serum, were compared regarding the effect of the lid. The type of culture medium did not affect the results. Production of CO_2 during the culture period did not affect the CO_2 concentration of the atmosphere of the culture flask, suggesting that the phenomenon was not due to the CO_2 concentrations. The explanation of the result may be as follows: A highly volatile growth regulating factor(s) was secreted from the cells, acting as either promotive or inhibitory depending upon the responsiveness of the cell types.
社団法人日本動物学会の論文
1991-02-15