Sperm-Egg Binding Mediated by Sperm α-L-Fucosidase in the Ascidian, Halocynthia roretzi(Biochemistry)

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概要

• 論文の詳細を見る

• Spermatozoa bind to the vitelline coat in the ascidians and many other animals. The binding of sperm in Halocynthia roretzi is mediated by a sperm α-L-fucosidase and complementary-L-fucosyl residues of glycoproteins in the vitelline coat. cDNA clones for α-L-fucosidase were isolated from growing testis mRNA. It contained a 1398 bp full-length cDNA insert (HrFuc'ase) that encoded the 466 amino acid residues of H. roretzi sperm α-L-fucosidase. A putative signal peptide of 21 amino acid residues proceeded the sequence for the mature protein (M. W. 52.4 kDa). The coding sequence for HrFuc'ase showed 47.7% sequence identity to the human liver fucosidase sequence. The polyclonal antibody was prepared against a lacZ-HrFuc'ase fusion protein expressed in E. coli. The antibody crossed to a 54 kDa protein in sperm on western blotting and inhibited fertilization in a dose dependent manner. These data suggest that spermegg binding is mediated by the sperm α-L-fucosidase, HrFuc'ase in the ascidian, H. roretzi.

• 社団法人日本動物学会の論文
• 2002-01-25

著者

• Matsumoto Midori

  Department Of Biological Sciences And Informatics Keio University

• Matsumoto Midori

  お茶の水女子大学 発生生物学

• Matsumoto Midori

  Department Of Biosciences And Informatics And Center For Life Science And Technology Keio University

• Matsumoto M

  Department Of Biosciences And Informatics And Center For Life Science And Technology Keio University

• Matsumoto Midori

  Department Of Biosciences And Informatics Faculty Of Science And Technology Keio University

• Matsumoto Midori

  Center for Life Science and Technology, Graduate School of Science and Technology, Keio University

• Hirata Junko

  Center for Life Science and Technology, Graduate School of Science and Technology, Keio University

• Hirohashi Noritaka

  Center for Life Science and Technology, Graduate School of Science and Technology, Keio University

• Hoshi Motonori

  Center for Life Science and Technology, Graduate School of Science and Technology, Keio University

• Hirata Junko

  Center For Life Science And Technology Graduate School Of Science And Technology Keio University

• Hoshi Motonori

  The University Of The Air

• Hoshi Motonori

  Department Of Biosciences And Informatics And Center For Life Science And Technology Keio University

• Hoshi Motonori

  Center For Life Science And Technology Graduate School Of Science And Technology Keio University

• Hoshi Motonori

  Department Of Life Sciemce And Technology Tokyo Institute Of Technology

• Matsumoto Midori

  Center For Life Science And Technology Graduate School Of Science And Technology Keio University

• Hirohashi N

  Department Of Biology Ochanomizu University

• 星 元紀

  The University Of The Air

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