Transmembrane Cell Signaling for the Initiation of Trout Sperm Motility : Roles of Ion Channels and Membrane Hyperpolarization for Cyclic AMP Synthesis(Reproductive Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Motility of trout sperm is suppressed by extracellular K^+ at rest and initiated by the decrease in the extracellular K^+ concentration, and Ca^<2+> and cyclic AMP participate in the cell signaling for the initiation of sperm motility. We showed here that tetraethyl ammonium (TEA) and some blockers used as the voltage-dependent K^+ channel blockers in the neuron inhibited the motility in the sperm of the rainbow trout and the steelhead trout, Oncorhynchus mykiss. Other types of K^+ channel blockers, e. g., apamin, a blocker of small conductance Ca^<2+>-dependent K^+ channels, did not inhibit sperm motility. L-type Ca^<2+> channel blockers such as nifedipine, nimodipine, etc. also inhibited the motility, but other types of Ca^<2+> channel blockers did not. On the other hand, the gradual increase in external K^+ concentration caused both gradual decreases in the amplitude of hyperpolarization of the sperm plasma membrane and cyclic AMP synthesis. TEA and nifedipin suppressed both hyperpolarization and cyclic AMP synthesis, and these suppressions were relieved by addition of the K^+ ionophore, valinomycin. The calmodulin inhibitors W-7, trifluoroperazine and calmidazol-Cl inhibited the sperm motility, membrane hyperpolarization and cyclic AMP synthesis, although only at rather high concentrations. These results suggest that K^+ efflux through K^+ channels and Ca^<2+> influx through Ca^<2+> channels that are sensitive to specific channel blockers cause the changes of membrane potential and lead to a synthesis of cyclic AMP in the cell signaling for the initiation of trout sperm motility.
- 社団法人日本動物学会の論文
- 2001-09-20
著者
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Oka Yoshitaka
Misaki Marine Biological Station Graduate School Of Science The University Of Tokyo
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Inaba K
Tohoku Univ. Aomori Jpn
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Inaba Kazuo
Asamushi Marine Biological Station Faculty Of Science Tohoku University
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Inaba Kazuo
Asamushi Marine Biological Station Graduate School Of Science Tohoku University
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Morisawa Masaaki
Misaki Marine Biological Station, The University of Tokyo
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Kho K
Misaki Marine Biological Station Graduate School Of Science The University Of Tokyo
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Kho Kang
Misaki Marine Biological Station, Graduate School of Science The University of Tokyo
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Tanimoto Satomi
Department of Biology, Faculty of Science, Toho University
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Tanimoto S
Department Of Biology Faculty Of Science Toho University
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Tanimoto Satomi
Department Of Biology Graduate School Of Science The Toho University
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Morisawa Masaaki
Misaki Marine Biological Station Graduate School Of Science The University Of Tokyo
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Tanimoto Satomi
Department Of Biology Faculty Of Science Toho University
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Oka Yoshitaka
Misaki Marine Biological Station Grad. Sch. Sci. Univ. Of Tokyo
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