Cell Structure of the Infectious Form of Holospora, an Endonuclear Symbiotic Bacterium of the Ciliate Paramecium(Cell Biology)

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Under a phase-contrast microscope, the infectious form of Holospora obtusa appears to consist of three regions in tandem : a dark region, a refractile region, and a small dark tip in the refractile region. By electron microscopy, this bacterium appears to consist of a cytoplasmic region, a periplasmic region, and a small electron-translucent tip in the periplasmic region. It had previously been thought that these three regions in each microscopy respectively corresponded to one another. However, indirect immunofluorescence microscopy with monoclonal antibodies, IF-3-1 and IF-3-2, specific for periplasmic proteins of H. obtusa and an antiserum raised against a cytoplasmic stress protein, the GroEL homologue of aphid endosymbiotic bacterium, showed that the dark region was the periplasm and the refractile region was the cytoplasm. This shows that the small dark tip does not correspond to the small electron-translucent tip, and that the electron-translucent tip should be present at the opposite end. We found that this special tip could be identified with Nomarski- and phase-contrast optics at the end of the dark region when the infectious form was embedded in 30% gelatin. The same correspondences between the light and the electron microscopic structures were also confirmed in the infectious form of the micronucleus-specific bacterium H. recta.
社団法人日本動物学会の論文
1999-02-15