Partial Characterization and Cryopreservation of the Isolated Symbiotic Bacterium Holospora recta of the Ciliate Paramecium caudatum

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概要

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• Holospora recta is a micronucleus-specific symbiotic bacterium of the ciliate Paramecium caudatum. This bacterium cannot grow outside the host cell. We isolated the infectious form of this bacterium from the host homogenates by 70% Percoll density gradient centrifugation. DNA in the infectious form of H. recta appeared as a large cluster in the cytoplasmic region near the large periplasm. This was not observed with the infectious form of a macronucleus-specific symbiont of P. caudatum, H. obtusa. The isolated infectious form could infect the micronucleus of P. caudatum and differentiated into the reproductive form within 18 hr after the infection at 25℃. However, not only was this bacterium unable to infect the micronucleus of P. bursaria, but it was also unable to infect the micronucleus of P. multimicronucleatum or P. novaurelia, even though the latter two species are morphologically closely related to P. caudatum. We succeeded in the cryopreservation of this bacterium, as cells stored at -85℃ for 127 days maintained their infectivity and reproducibility.

• 社団法人日本動物学会の論文
• 1996-10-15

著者

• Kawai M

  Yamaguchi Univ. Yamaguchi Jpn

• Kawai Miki

  Biological Institute Fuculty Of Science Yamaguchi University

• Fujishima Masahiro

  Biological Institute, Fuculty of Science, Yamaguchi University

• Fujishima M

  Yamaguchi Univ. Yamaguchi Jpn

• Fujishima Masahiro

  Biological Institute Faculty Of Science Yamaguchi University

関連論文

• ENDONUCLEAR SYMBIOTIC BACTERIUM HOLOSPORA SPECIES DISTINGUISHES THEIR TARGET NUCLEAR ENVELOPES(Taxonomy and Systematics & Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Partial Characterization and Cryopreservation of the Isolated Symbiotic Bacterium Holospora recta of the Ciliate Paramecium caudatum
• DYNAMICS OF A 89K PROTEIN LOCALIZING AT A SPECIALIZED TIP OF ENDONUCLEAR SYMBIOTIC BACTERIUM HOLOSPORA IN INFECTION(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• INFECTION OF ENDOSYMBIOTIC BACTERIUM HOLOSPORA OBTUSA DEPRESSES SURFACE ANTIGEN GENE EXPRESSION OF THE HOST PARAMECIUM CAUDATUM(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Cell Structure of the Infectious Form of Holospora, an Endonuclear Symbiotic Bacterium of the Ciliate Paramecium(Cell Biology)
• THE SPECIFIC BINDING BETWEEN OUTER MEMBRANE SUBSTANCES OF THE ENDONUCLEAR SYMBIOTIC BACTERIUM HOLOSPORA AND THE HOST CHROMATIN(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• PROPERTIES OF MICRONUCLEAR CHROMATIN-SPECIFIC ANTIGEN OF PARAMECIUM CAUDATUM(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Host-Symbiont Interactions in Paramecium-Chlorella Symbiosis(Invertebrate-Algal Symbiosis: Diversity and Evolution,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
• Interaction between endonuclear symbiotic bacterium Holospora and its host Paramecium(Symposium on Various Interactions between Animal and Microorganism)
• Light and electron microscopic observations of Holospora obtusa--A macronucleus-specific bacterium of the ciliate Paramecium caudatum

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