Effects of DIF-1, an Anti-Tumor Agent Isolated from Dictyostelium discoideum, on Rat Gastric Mucosal RGM-1 and Leptomeningeal Cells(Cell Biology)

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概要

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• DIF-1 is a putative morphogen that induces stalk cell formation in the cellular slime mold Dictyostelium discoideum. We have previously discovered that DIF-1 suppresses cell growth and induces cell differentiation in vitro in some tumor cells, and also that relatively low concentrations of DIF-1 promote retinoic acid-induced cell differentiation in the human myeloid leukemia HL-60 cells. In this study, to verify cell biological and therapeutic potential of DIF-1, we have examined whether and how DIF-1 affects normal mammalian cells in vitro, using rat leptomeningeal (RLM) cells and rat gastric mucosal RGM-1 cells. In growing phase of both cells, DIF-1 at 5-40μM suppressed cell growth in a dose-dependent manner. High concentrations (15-40μM) of DIF-1 were toxic to the growing cells so that the cells showed unusual morphology, but many of them were still alive even at Day 3-4. Withdrawal of DIF-1 allowed the cells to grow. In confluent phase of the cells, DIF-1 at more than 15μM promoted medium acidification that resulted in cell death, but DIF-1 itself did not markedly affect cell viability for 3 days. DIF-1 increased [Ca^<2+>]_I, in RLM cells but did not affect β-trace secretion (an index of cell function in RLM cells). A low concentration (5μM) of DIF-1 in combination with retinoic acid (0.1μM) showed no marked effects on cell growth, cell viability, cell morphology and p-trace secretion in RLM cells. The present results indicate that DIF-1 may be utilized as a tool for cell biology. Also, since these concentrations of DIF-1 kill some tumor cells within 2-3 days, the present results suggest that DIF-1 might be utilized in the treatment of some sorts of cancer with a bearable degree of side-effects.

• 社団法人日本動物学会の論文
• 1998-10-15

著者

• Matsui Hirofumi

  Institute Of Physical And Chemical Science (riken) Cell Bank:institute Of Clinical Medicine Universi

• Kubohara Y

  Gunma Univ. Maebashi Jpn

• Kubohara Yuzuru

  Department Of Molecular Physiology Institute For Molecular And Cellular Regulation Gunma University

• Ishikawa Koichi

  Department Of Molecular Physiology Institute For Molecular And Cellular Regulation (imcr) Gunma Univ

• Ishikawa Koichi

  Department Of Industrial Chemistry Faculty Of Technology Tokyo Metropolitan University

• Kabeya Kenji

  Department of Molecular Physiology, Institute for Molecular and Cellular Regulation (IMCR), Gunma Un

• Kabeya Kenji

  Department Of Molecular Physiology Institute For Molecular And Cellular Regulation (imcr) Gunma Univ

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