Saturation Density of Tetrahymena with or without Agitation at Various Surface-to-Volume Ratios of Culture Test Tubes(COMMUNICATION)(Cell Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Saturation density of Tetrahymena pyriformis in a test tube without shaking can be greatly enhanced, if a small amount of culture medium is layered on top of solid agar containing the same culture medium in the test tube so that the ratio of area of airmedium interface to volume is a large one. Growth of Tetrahymena pyriformis in such cultivating tubes with and without shaking was investigated under various conditions of the surface-to-volume ratio. The saturation density of the cells reached 1 × 10^8 cells/ml in the tube with the largest surface-to-volume ratio (39cm^<-1>) without shaking. When such a tube was shaken during cultivation, the saturation density of the cells was significantly lower than that without shaking. T. pyriformis was also cultivated in a test tube with a small surface-to-volume ratio such that the oxygen supply was insufficient. The saturation density of the cells in such a tube of small surface-to-volume ratio with shaking was slightly higher than that without shaking. These results suggest that the saturation density of the cells in a tube of large surface-to-volume ratio is markedly restricted by slight mechanical agitation (shaking).
- 社団法人日本動物学会の論文
著者
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Asai Hiroshi
Department Of Bioscience And Biomedical Engineering Graduate School Of Science And Engineering Wased
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Asai Hiroshi
Department Of Physics School Of Science And Engineering Waseda University
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SAITOH TADAO
Department of Physics, School of Science and Engineering, Waseda University
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Saitoh Tadao
Department Of Physics School Of Science And Engineering Waseda University
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