Paramecium caudatumの分裂間期に於ける核酸代謝の研究

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概要

• 論文の詳細を見る

• The aim of this study is to elucidate the metabolism of RNA and DNA during the interphase of Paramecium caudatum. The stock culture was transferred into the physiologically balanced solution containing ^<32>P. After incubation at 25℃ for 1.5 hours, organisms were fixed, a series of histological sections being prepared. The preparations were then treated with RN-ase or DN-ase to remove RNA or DNA respectively. The microautoradiograms were made by means of stripping method and then the numbers of the tracks were counted. Meanwhile the nucleic acid contents in macro- and micronuclei as well as the cytoplasm were determined after the differential centrifugation. The result of chemical analysis revealed that little RNA was detected in micronuclei whereas both nuclei contained an appreciable amount of DNA. However DNA was found metabolically inactive throughout the interphase. On the contrary, ^<32>P was incorporated into both RNA found in macronuclei and cytoplasm actively, especially during intermediary stage from daughter cells to the prefission stage.

• 社団法人日本動物学会の論文
• 1960-07-15

著者

• 重松 昭世

  東京大学医学部衛生看護学科

• 重松 昭世

  東大・医・衛看・生化

関連論文

• 綜合討論(発生学・実験形態学)
• ミクロオートラジオグラフによる絹糸せんの核酸およびタンパク代謝の研究
• Paramecium caudatumの分裂間期に於ける核酸代謝の研究
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• ムラサキウニの卵割に及ぼすアズレノイド化合物の影響(予報)(発生学・実験形態学)

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