ミクロオートラジオグラフによる絹糸せんの核酸およびタンパク代謝の研究

概要

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1. A semiquantitative microautoradiographic technic was applied to the metabolic studies of protein as well as ribonucleic acids in the posterior silkglands during the fifth instar period of Bombyx mori. 2. Actually no incorporation of ^<32>P or ^<14>C-glycine into deoxyribonucleic acid was observed. Whereas, a remarkable renewal of RNA-^<32>P was shown around 2nd and 3rd days of the fifth instar. Later the incorporation of ^<14>C-glycine into fibroin began from the 3rd day and reached to a maximum at the 7th day. These observations led to the conclusion that the co-synthesis of ribonucleic acid during the fibroin biosynthesis is not required. 3. Different sites of the incorporation of ribonucleic acid were observed in the different labeled compounds : ^<32>P was incorporated rather in external area of silkglands, whereas ^<14>C-glycine was incorporated solely into the nuclear RNA. Both labeled compounds did not enter into the RNA-core after RN-ase treatment.
日本応用動物昆虫学会の論文
1960-06-30