位相差顕微鏡による異種移植の腫瘍細胞の観察

概要

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The course of the degeration process of tumor cells of Yoshida sarcoma, MTK-sarcoma II and MTK-IV tumor, after the heteroplastic transplanation into the mouse peritoneal cavity, was studied in the living condition by phase-contrast microscopy using hanging drop preparations made in combination of fluid paraffin. The tumor cells after inoculation in the peritoneal cavity of mice can survive for 5 to 6 days (sometimes 7 days) undergoing a considerable proliferation with the accumulation of the ascites in a remarkable degree. But they then rapidly disappear without killing hosts. The process of degeneration of tumor cells was studied with the samples taken at a 2-hour-interval at tha time of disappearance. The tumor cells being in a pure culture condition at the 5th to 6th day after transfer suddenly undergo degeneration : they show remarkable granular bodies and small vacuoles in cytoplasm. Then these cells begin to disintegrate with the irregular transformation of cell-form following the formation of picnotic nuclei. The decrease in number of tumor cells in the peritoneal cavity is followed by the increase of the number of blood cells such as macrophages and lymphocytes. There are still survival small-sized tumor cells in the peritoneal cavity, and meanwhile they are all damaged by phagocytic leucocytes.
社団法人日本動物学会の論文
1957-05-15