カエルに投與した^<32>Pの分布とその經過

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We have studied the distribution of administered radiophosphorus in various organs of Rana nigromaculata nigromaculata Hallowell, preceeding application of radiophorus to our studies on the phosphorus metabolism of photoreceptor. The experiments were carried out in summer (July, 1950) and in winter (March, 1952). Ringer's solution containing radioactive disodium phosphate (about 60 μ C/ml) was intraperitoneally injected in the proportion of 0.01ml per gram body weight. Five frogs were dissected every hours after administration, and in each case the measurement of radioactivity was performed on the samples obtained from eyes, blood, liver, muscle, bone and brain. The results are as follows: 1. With lapse of time the labeled phosphorus accumulates gradually in the eyes as well as in the brain, being led to maximum uptake about 36 hours after administration (See Fig.1). In winter the maximum retention by eyes 0.25% of administered radiophosphorus is markedly higher than in summer (See Table I). 2. Table II shows the distribution of radiophosphorus in various tissues. The maximum retentions per unit fresh weight of tissue decrease in the following order: liver, bone, muscle, brain, eye and blood. The content of radiophosphorus in the blood reaches maximum within one hour after administration, and then its rapid decrease takes place. The rate of decrease is more remarkable in winter, and the concentration is maintained after 24 hours on about half as high as in summer. The radioactivity of muscle and brain increases very gradually even 120 hours after injection of radiophosphorus. The muscle of winter frog retains radiophosphorus twice as much as that of summer frog. In the liver which exhibites highest activity amoung tissues, the maximum uptake appears 2-3 days after administration. The deposition in bone is noticeable with the exception of liver. 3. The most remarkable difference between summer and winter frog is that the tissues of the latter take up more amounts of radiophosphorus for the given time after administration, while the blood is more quickly deprived of its activity. The reason may lie in seasonal and other physiological conditions of the experimental animals. Thus we come to the conclusion that the winter frog metabolizes phosphates rather actively than the summer one, though we can not leave out of consideration that the winter frog used in our experiments was about to become active withina month, being somewhat different conditions from those in the midst of hibernation. 4. In general the uptake of radiophosphorus by various tissues of a cold-blooded animal (frog) does not strikingly differ from that of a warm-blooded animal (rat, mouse). Some remarkable points in the frog are: the retention by liver is higher than by bone, the activity in blood disappears quickly, the maximum uptake by liver is found earlier than in mouse, and so on. The muscle of frog seems to resemble that of mouse, indicating the maximum uatake 5 days after injection. The lasting accumulation of radiophosphorus observed in bone of rat and mouse can not be clearly recognized in our experiments. The results described above are necessarily concerned with the distribution of labeled phosphorus retained among different phosphorus compounds of the tissues. The details of referring to distribution in eye-tissues will be reported in future.
社団法人日本動物学会の論文
1953-02-15

カエルに投與した^<32>Pの分布とその經過

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