生物変異の光力学的誘発I.表現型模寫
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Larvae of Drosophila melanogaster from the isogenic Oregon strain aged from 50 to 100 hours after hatching, were examined for induction of phenocopies by 0.01 per cent illuminol RII (3・3′・3″・4・4′・4″-hexameteyl-7-(2″-methyl thiazolyl)-2.2′-trimethin-thiazolocyanin-3・3″-dijodid) and visible light. The absorption spectrum of this dye solution is illustrated in fig. 1. As shown in table 1-I, more than two thousands of larvae were exposed for 5 hours to the beam of 100 watt projection lamp through a water bath and a Matsuda VR3 filter which is translucent more than 600mμ of wave length. After this treatment about 78 per cent of larvae became flies, and of them about 6 per cent revealed abnormalities. Most abnormalities (more than 90 per cent) occurred on proboscis, and many of them resembled proboscipedia. Other than proboscipedia abnormal abdomen, missing bristle, folded wing etc. were also appeared. In the present case the peak of the effect appears only around 70 hours age, and there are fairly sharp decreases before and after this point. As shown in table 1-II larvae of 70 hours old were immersed in dye solution and kept in darkness without irradiation through the following 7 hours, then transferred to usual media, to allow their emergence. Only very few flies thus emerged showed abnormalities. Then larvae treated with dye solution of illuminol RII were exposed to light through a water bath and a VB1 filter (translucent 400-600mμ; the maximum is in 480mμ). Thus emerged flies revealed no abnormolity as well in the case as the larvae were irradiated only by the visible light without dye treatment. These results seem to indicate that the abnormalities in table 1-I were induced by the photodynamic action, and the active part of the wave length seems present in 600mμ. It is known that the illuminol RII contains a small amount of trace elements. So vitacyanin A, B, C and D were tested for their photodynamic action. Although vitacyanin has the same chemical composition as illuminol RII, in the degree of contents of above trace elements, it differs from illuminol RII. That is, vitacyanin D is the most pure, containing nearly no trace elements with gradual increse from C to A. As shown in table 1-III, no indication of relation between the induction abilities and the trace elements was seen. Acridin orange, eosin and erythrosine were also tested for their abilities to induce phenocopies. But as seen in table 1-IV, eosin and erythrosine induced no abnormalities, except acridin orange, but this occurs both under irradiation and darkness. Thus the induction ability of acridin orange is difficult to attribute to photodynamic action.
- 社団法人日本動物学会の論文
- 1953-12-15
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