両生類の骨髄細胞の染色体観察法
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概要
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The chromosome preparation method of Tjio and Whang (1962) for higher vertebrates was modified for use in lower vertebrates. Bone marrow from the frog femur and tibia was aspirated and suspended in amphibian Ringer's solution containing colchicine (5 μg/ml) for 2 hours at 20-26℃. Cells were then treated with distilled water for 15 minutes, fixed with acetic alcohol (1: 3), and centrifuged. The cells were refixed with fresh fixative for 30 minutes prior to drying the cells on slides, and then stained with Giemsa. The method described facilitates the analysis of chromosome complements in adult male and female frogs regardless of season and age of animals.
- 社団法人日本動物学会の論文
- 1965-07-15
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