ウニ卵電極刺入の簡便法

概要

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An improved device of the microelectrode impalement into a sea urchin egg consists of an oscillatory plate of an electromagnetic speaker, which acts as the electro-mechanic transducer and is attached to the upper face of the electrode holder. Current pulses are supplied to the voice coil of the magnet at the frequency of 70-100Hz. In this way the tip of the microelectrode vibrates longitudinally. The amplitude and the duration of vibration should be changed according to the condition of eggs to be penetrated. When the tip of microelectrode is pushed into the cell during continued vibration, membrane potential can be usually measured as a result of successful impalement. It is also possible to demonstrate the change of membrane potential upon fertilization in a Pseudocentrotus egg by this method.
1971-05-15