ウニ卵における付活後の膜電位変化と発生能力との関係

概要

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Changes in the membrane potential upon activation of a sea urchin (Hemicentrotus pulcherrimus) egg were examined in relation to the process of development. The methods of activation were 1) normal fertilization, 2) treatment with butyric acid-sea water, 3) treatment with butyric acid-sea water followed by hypertonic sea water treatment. The membrane potentials of unfertilized eggs were -12 to -16 mV. The membrane showed a transient depolarization (10-16 mV) closely associated with the elevation of the fertilization membrane due to activation, followed by polarization up to a steady level of -68 to -80 mV. Magnitudes of the initial depolarization and the subsequent hyperpolarization of the egg activated by butyric acid-sea water were lower than those of normally fertilized eggs. When the butyric acid-treated eggs were then treated with hypertonic sea water, the magnitude of the hyperpolarization became almost the same as that of normally fertilized eggs. The eggs treated with butyric acid only were unable to develop further than the morula stage at the best, while the eggs subaequently treated with hypertonic sea water showed nearly normal development up to the prizm stage. The magnitude of membrane potential of sea urchin eggs after activation was found to be correlated with its developmental activity.
社団法人日本動物学会の論文
1971-10-15