高速度映画による繊毛運動の撮影

概要

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A method for recording the movement of a large abfrontal cilium of Mytilus edulis with a high-speed microcinematography was described. As shown in Fig. 1, the cilium is illuminated by an intermittent light which synchronizes with the shutter of a high-speed cinecamera (HS). The image of the cilium is projected on the film on the camera after a magnification of 50 diameters with a 40× phase-contrast objective, a 5× ocular and a relay lens (L_4, f=3.125cm). The light emitting from a super high-pressure mercury arc (ML) throws an image of a slit (S) inserted into the light path on the level of the cilium (Cil) under the objective lens. When the mirror (M_2) rotates, the image of the slit rapidly moves across the field of the microscope, so that the cilium is illuminated for a brief period. The duration of the illumination, which depends on the crank-speed of the camera, is 1/20, 000 second when the crank-speed is 500 frames per second. The light intensity is sufficient for forming images of good quality on plus-X films in the above condition, while neither the effect of heating nor other injurious effects of illumination on the ciliary movement are detected. The results are shown in Figs. 3, 4 and 5.
社団法人日本動物学会の論文
1970-01-15