ウニ卵の受精とグルコース-6-リン酸デヒドロゲナーゼの解離(予報)

概要

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In sea urchin eggs, glucose-6-phosphate dehydrogenase (G6PDH) is released upon fertilization (Isono, 1963b). In the present experiment, the time course of the release was studied in eggs of Anthocidaris crassispina. After egg homogenate in 0.75M mannitol was centrifuged, G6PDH activity was determined in the soluble and the particulate fractions, and the percentage of soluble G6PDH was calculated. As in Fig. 1, only 7% of the total activity was found in the soluble fraction in unfertilized eggs, while the amount of soluble enzyme began to increase about 75-90 seconds after fertilization, and 70% or more became soluble in 3 minutes. The release of G6PDH begins about 30 seconds later than the TPN increase, but is essentially similar in timing to the changes of the G6P level and of oxygen uptake (Epel and Iverson, 1965). Thus the time course of G6PDH release agrees well with the speculation that the G6PDH release at fertilization is brought about by the increases of TPN and G6P (Isono and Yasumasu, 1968)
社団法人日本動物学会の論文
1969-08-15