胸腺細胞の放射線死における活性分子の役割

概要

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It has been previously shown that certain sulfhydryl group (SH)-reactive molecules are formed from some nucleic acid constituents or amino acids by X^irradiation (Akita, Ueno and Shima, 1966; Ueno and Akita, 1967). In the present study the survival of unirradiated thymocytes was found to decrease during the incubation in vitro with some nucleosides or histidine which were irradiated previously with γ-rays in Ringer solution. When the doses (8-204kR) of irradiation of histidine were increased, the cells died faster. There is however, always a time elapse more than 30 min from the beginning of incubation to the first appearance of dead cells. Over the dose-range of 8-26kR, survival was independent of the dose. The addition of cysteine, a thiol protective compound, to the irradiated histidine solution 15 min prior to mixing it with thymocytes suspension abolished completely the cytocidal effect of the irradiated histidine. On the other hand, the effect was prevented only partially when cysteine was added to thymocytes suspension and then the irradiated histidine solution was added to it 15 min later. These results seemed to suggest that the SH-reactive molecules formed from some nucleosides or amino acids by irradiation played a role in the process leading to the radiation death of thymocytes.
社団法人日本動物学会の論文
1968-11-15