The Distribution of Dividing Endothelial Cells of Chick Embryonic Aortae Studied by Vital Labeling with Bromodeoxyuridine

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概要

• 論文の詳細を見る

• The proliferation of aortic endothelial cells of 10, 14, and 20 day-old embryonic and 3 day post hatch chicks was studied by vital labeling with bromodeoxyuridine(BrdU)and immunofluorescent visualization using anti-BrdU. We modified the DNA denaturation step using 0.2% Triton X-100 and incubation with 0.1M hydrogen chloride for 15min at 30 to 35℃. These modifications were effective to stain the chromosomes with anti-BrdU in the whole mounted tissues. The BrdU-positive endothelial cells were present in the whole aortic areas without showing any mitotic hot spots. The average labeling index of the endothelial cells was high in the young stages ; 3.7% at 10 day-old embryos, and then it reduced to 1.4% at 3 day-old chicks.

• 社団法人日本動物学会の論文

著者

• NOMURA TAKAHIRO

  Department of Fermentation Technology, Faculty of Engineering, Yamanashi University

• Nomura Takahiro

  Dept. Bio. Fac. Sci. Chiba Univ.

• Jinguji Yoichi

  Gunma Prefectural College of Health Sciences

• Jinguji Y

  Gunma Prefectural Coll. Of Health Sci. Gunma Jpn

• Nomura Takahiro

  Department Of Fermentation Technology Faculty Of Engineering Yamanashi University

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