Evidences for Direct Involvement of Microtubules in Cleavage Furrow Formation in Newt Eggs

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概要

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• This paper aims at examining the effect of colchicine, a microtubular poison, on the process of furrow formation in whole eggs and egg fragments as well as the process of artificial induction of furrow-like dents, in eggs of the newt, Cynops pyrrhogaster. To apply colchicine locally to eggs, the eggs were slit across or along a furrow in a colchicine solution during first cleavage. When a slit was made across or in front of a growing furrow at the onset of its growth, the furrow quickly ceased growing and often regressed. Cortices containing an entire growing furrow were isolated along with a thin layer of subcortical cytoplasm immediately after the start of the first cleavage. Furrows in the cortices degenerated when the cortices were cultured in a colchicine solution, whereas they continued growing when they were cultured in Holtfreter's saline. Furrow-inducing cytoplasm was injected to a site beneath the cortex in the animal half of the egg during first cleavage. When a small slit was made close to the site of the injection in a colchicine solution, no furrow-like dent was induced. These results imply that microtubules are directly involved in the generation and growth of cleavage furrows.

• 社団法人日本動物学会の論文

著者

• Sawai Tsuyoshi

  Department Of Biology Faculty Of General Education Yamagata University

関連論文

• Cytostatic Effect of the Cytoplasm of Mature Oocytes in the Newt, Cynops pyrrhogaster : Developmental Biology
• Movement of the Cell Surface in the FirstCleavage Stage in the Newt Egg
• Surface Movement in the First Cleavage Stage of Newt Eggs(Developmental Biology)
• The Effect of Colchicine on Progress of Cleavage Furrow inAmphibian Eggs
• Effect of protein phosphatase inhibitors on cleavage furrow formation in newt eggs: Inhibition of normal furrow formation and concomitant induction of furrow like dents
• Furrow-inducing Activity of Extra Sperm NucleiPresent in Egg Fragment without Zygote Nucleus in Newt Eggs
• Microinjected Ca2+ Stores with Inositol 1,4,5-Trisphosphate Recepror to Dividing Newt Eggs Moves Toward the Presumptive Cleavage Furrow Together with F-Actin in a Microtubule- and ATP-Dependent Manner(Cell Biology and Morphology)Proceedings of the Seventy
• Evidences for Direct Involvement of Microtubules in Cleavage Furrow Formation in Newt Eggs
• Microscopic Observation of Surface Relaxation and Contraction Waves in Elongated Eggs of the Newt during Cleavage(Developmental Biology)

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