Changes in Phosphorylation Activities during Goldfish and Xenopus Oocyte Maturation
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概要
- 論文の詳細を見る
Oocyte maturation is promoted by the sequential actions of several kinases, of which MPF(a histone H1 kinase) and MAP kinase (a myelin basic protein (MBP) kinase) are known to play pivotal roles. However, other kinases responsible for inducing oocyte maturation have yet to be characterized. To identify these kinases, we examined phosphorylation activities toward 44 exogenous substrate proteins during oocyte maturation in goldfish and Xenopus. We found that 4 substrates in goldfish and 6 in Xenopus were phosphorylated and their phosphorylation states changed during oocyte maturation. Among them, only 3 substrates (histone H1, MBP and pepsin) were common to both species. Precipitation of cdc2 showed that, like histone H1, pepsin was also phosphorylated by cdc2 (MPF). These results suggest that different kinase cascades are involved in goldfish and Xenopus oocyte maturation, although MPF and MAP kinases are common to both species. We also found novel phosphorylation activities that precede the activation of MPF and MAP kinases using deoxyribonuclease I, casein, pepsin and protamine as exogenous substrates.
- 社団法人日本動物学会の論文
- 1995-10-15
著者
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Yamashita Masakane
Laboratory of Molecular and Cellular Interactions, Graduate School of Science, Hokkaido University
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Yoshida Noriyuki
Laboratory Of Molecular And Cellular Interactions Divivion Of Biological Sciences Graduate School Of
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Yoshida Noriyuki
Laboratory Of Molecular And Cellular Interactions Division Of Biological Sciences Graduate School Of
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Yamashita Masakane
Laboratory Of Molecular And Cellular Interactions Division Of Bioloaical Sciences Graduate School Of
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Yamashita Masakane
Laboratory Of Molecular And Cellular Interactions Divivion Of Biological Sciences Graduate School Of
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Tanaka Toyomi
Laboratory of Molecular and Cellular Interactions, Divivion of Biological Sciences, Graduate School
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Tanaka Toyomi
Laboratory Of Molecular And Cellular Interactions Divivion Of Biological Sciences Graduate School Of
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