Cardiotoxic Effects of Adrenochrome and Epinephrine on the Mouse Cultured Myocardium

概要

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Effects were studied on cultured mouse ventricular myocardial cells of 10<-6>-10<-3>M epinephrine and adrenochrome, a metabolite of epinephrine. Single ventricular myocardial cells in culture had negative chronotropic responses to adrenochrome (10<-4>M) and positive responses to epinephrine (10<-6>M). However, cultured ventricular myocardial cells in confluent sheets had positive chronotropic responses to adrenochrome (2×10<-4>M) and epinephrine (10<-6>M). Cumulative LDH leakage from ventricular myocardial cells in confluent sheets increased remarkably after application of 10<-3>M adrenochrome but only somewhat after application of l0<-3>M epinephrine. Both adrenochrome and epinephrine (10<-6>M) induced mitochondrial fusion and an increase of atrial specific granules, in ventricular myocardial cells in confluent sheets. Epinephrine induced an increase in size of myofibrils. In cultured ventricular myocardial cells, adrenochrome at 2×10<-4>M induced mitochondrial damages, as shown by a swollen appearance, but epinephrine never induced such necrotic changes even at concentrations up to 10<-3>M. The role of adrenochrome and epinephrine in catecholamine cardiotoxicity is discussed.
社団法人日本動物学会の論文
1993-06-15