Mouse γ-Casein cDNA : PCR Cloning and Sequence Analysis
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概要
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A partial amino acid sequence of mouse γ-casein was determined on a gas-phase amino acid sequencer. The N-terminal sequence (23 residues) was obtained using native γ-casein, and the C-terminal sequence (13 residues) was determined with a corresponding peptide. The C-terminal peptide was isolated by affinity chromatography on an anhydrotrypsin agarose column after digestion of γ-casein with Achromobacter lysyl-endopeptidase. A cDNA (507 base pairs) encoding the mature protein of γ-casein was produced by polymerase chain reaction (PCR) amplification of lactating mammary gland first strand cDNA with oligonucleotide primers designed from the N-terminal (KHEIKDK-) and the C-terminal (-AHYTRFY) amino acid sequences. The full-length cDNA including 5'- and 3'-noncoding regions (920 base pairs) was cloned by the rapid amplification of cDNA ends (RACE) method of Frohman et al. [7]. Comparison of full-length cDNAs of mouse γ-casein and rat γ-casein showed 80% identity at the nucleotide level. The amino acids in the coding region of both γ-caseins were 75% identical.
- 社団法人日本動物学会の論文
- 1993-02-15
著者
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Sasaki Masato
Research Laboratory Zenyaku Kogyo Co. Ltd.
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Sasaki Tetsuo
Research Laboratory Zenyaku Kogyo Co. Ltd.
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ENAMI JUMPEI
Research Laboratory, Zenyaku Kogyo Co., Ltd.
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Sasaki T
Kawasaki Medical School Okayama Jpn
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Enami Jumpei
Research Laboratory Zenyaku Kogyo
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Sasaki T
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Enami J
Zenyaku Kogyo Co. Ltd. Tokyo
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Sasaki M
Department Of Industrial & Information Systems Engineering Ashikaga Institute Of Technology
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Enami Junpei
Research Laboratory Zenyaku Kogyo Co Ltd
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Sasaki Masato
Research Laboratory Zenyaku Kogyo
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