Hormone-Dependent Expression of γ-Casein mRNA in Mouse Mammary Epithelial Cells Cultured on Floating Collagen Gels
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概要
- 論文の詳細を見る
Mammary epithelial cells isolated from pregnant mice were plated on a collagen gel matrix and cultured in a serum-free medium supplemented with combinations of insulin, dexamethasone and prolactin (PRL). After the cells formed a monolayer, the collagen gel was detached from the culture dish and allowed to float in the medium. Quantification of γ-casein mRNA by a competitive PCR method revealed that the cells on the floating gel accumulated considerably larger amounts of γ-casein mRNA than the cells on the gel remained attached to the dish. Under these floating collagen gel culture conditions, addition of both dexamethasone and PRL to the insulin-supplemented basal medium maximally stimulated the accumulation of γ-casein mRNA. These observations strongly suggest that the status of the extracellular matrix as well as hormones controls the differentiation of mouse mammary epithelial cells at the mRNA level.
- 社団法人日本動物学会の論文
- 1996-08-15
著者
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Sasaki Tetsuo
Research Laboratory Zenyaku Kogyo Co. Ltd.
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Sasaki T
Research Laboratory Zenyaku Kogyo Co. Ltd.
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ENAMI JUMPEI
Research Laboratory, Zenyaku Kogyo Co., Ltd.
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Enami Jumpei
Research Laboratory Zenyaku Kogyo
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Enami J
Zenyaku Kogyo Co. Ltd. Tokyo
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