Eurythermic Growth and Synthesis of Heat Shock Proteins of Primary Cultured Goldfish Cells : Cell Biology and Morphology

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Cells derived from the tail fin of goldfish (Carassius auratus) are cultured at 37℃ (GTF e-2) and 27℃ (GTF e-3). It is observed that the doubling times of GTF e-2 cells and that of GTF e-3 cells depend solely on the incubation temperature, irrespective of difference in the temperature at which the primary culture is started. It has been reported previously that some goldfish cell lines cultured in vitro for a long time grew stenothermically. The present study indicated that cells derived from the primary culture retained their ability to grow eurythermically at least in the early passages. The relationship of protein synthesis as to incubation temperature was studied empirically. When GTF e-3 cells were exposed to 37℃, four major heat shock proteins were induced. Their molecular weights were 90 K dalton (hsp 90), 70 K dalton (hsp 70), 42 K dalton (hsp 42) and 30 K dalton (hsp 30). In GTF e-2 cells these proteins were synthesized constitutively at 37℃. The levels of synthesis of these proteins were much higher than those observed when the cells were incubated at 27℃. At 40℃, hsp 70 and hsp 30 were the dominantly synthesized proteins of GTF e-2 and e-3 cells.
社団法人日本動物学会の論文
1990-06-15