Expression of Circular Plasmids Which Contain Bacterial Chloramphenicol Acetyltransferase Gene Connected to the Promotoer of Polypeptide IX Gene of Human Adenovirus Type 12 in Oocytes, Eggs and Embryos of Xenopus laevis : Cell Biology

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Polypeptide IX gene of adenovirus type 12 is unique in that it is expressed intermediately inbetween early and late genes, but the structure and function of its 5'-upstream promoter region have not been well characterized. In the present experiment, fertilized eggs as well as oocytes and unfertilized eggs of Xenopus laevis were injected with circular plasmid, pAdl2. IXCAT, which contains bacterial chloramphenicol acetyltransferase (CAT) gene fused to the promoter of polypeptide IX gene of adenovirus type 12, and the activity of this plasmid to promote CAT enzyme expression in Xenopus embryonic cells was examined. For comparison, pAd12.ElaCAT which contains the promoter of Ela protein of adnovirus type 12, pSV2CAT which contains SV40 early promoter, and pSV0CAT and pA10CAT3m which do not contain promoter were also tested. In the oocyte nucleus, all these circular plasmids were expressed similarly actively. In embryos and unfertilized eggs, however, while pAd12.ElaCAT and pSV2CAT were strongly expressed, level of the expression of pAd12. IXCAT was as low as those of pSV0CAT and pA10CAT3m. These results show that the promoter of polypeptide IX gene of adenovirus type 12 is very weak as compared with that of Ela protein.
社団法人日本動物学会の論文
1990-04-15