Effects of puromycin and α-amanitin on the activity of alkaline phosphatase in early preimplantation mouse embryos : COMMUNICATION : Developmental Biology

概要

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The activity of nonspecific alkaline phos-phatase (ALPase) was cytochemically and biochemically investigated in early preimplantation mouse embryos. The ALPase activity was cytochemically detected in embryos from 2- to 8-cell stage. The activity was exclusively localized on the adjacent cell membranes of the two blastomeres. This activity was biochemically first detected at the very low level in the 2-cell embryos. Biochemical assay revealed that in 4- to 8-cell embryos the ALPase activity increased dramatically. In these embryos, intense activity of ALPase was also detected cytochemically. When 2-cell embryos were treated with puromycin (15 ug/ml) in culture, the embryos could not develop to advanced stages and the expression of ALPase activity was inhibited. However, a-amanitin (2 and 10 pg/ml) suppressed the further cleavage , although it did not interfere with the expression of ALPase activity. The results suggest that mRNA molecules for ALPase of maternal origin exist in 2-cell embryos.
社団法人日本動物学会の論文
1990-02-15