CRITICAL ELECTROLYTE CONCENTRATION OF DNA AND NUCLEOPROTEIN COMPLEXES IN VITRO

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Critical electrolyte concentration (CEC) values were determined for DNA and DNA/H1 and DNA/protamine complexes under varying toluidine blue staining conditions and using Mg^<2+> as inorganic cation. CEC values were considered those molarities of MgCl_2 at which green staining was detected microspectrophotometrically. Variation in the CEC values was found with respect to association of nuclear proteins with DNA and the nucleoprotein complex considered. The DNA filaments exhibited the highest range of CEC values, whereas the lowest CEC values were presented by the DNA/protamine complex, especially when the staining solutions were prepared in McIlvaine buffer. The method thus appears to be promising for further studies on differences among DNA/protein complexes in vitro or even in situ.
日本組織細胞化学会の論文