POSTNATAL DEVELOPMENT OF PEROXIDASE ACTIVITY IN THE LARYNGEAL AND TRACHEAL EPITHELIA AND GLANDS OF SPECIFIC PATHOGEN FREE RATS

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The postnatal development of peroxidase (PO) activity in laryngeal and tracheal epithelia and glands was histochemically investigated in specific pathogen free (SPF) rats. Tissues were fixed in a cold 1% p-formaldehyde-1.25% glutaraldehyde solution in a 0.1 M sodium cacodylate buffer, pH 7.4, for 30 min at 4℃, and incubated in a diaminobenzidine-H_2O_2 medium including 2 mM KCN for 60 min at 37℃. The laryngeal glands had formed four days after birth, and PG activity was seen in some terminal tubule cells on day 6. Five days after birth, some tracheal epithelial cells invaginated into the matrix and formed glands, which became PG positive on day 11. The distribution of PG activity in the glands on day 14 resembled that of adult rats (3 months). The respiratory tract columnar epithelial cells of SPF rats were entirely devoid of the activity, although these cells started exhibiting positive activity when infected with some microorganisms such as corynebacteria. These results indicated that the glands of the rat respiratory tract areformed and synthesize PG after respiration has occurred, that regional difference seems to be important to gland maturation, and that respiratory tract columnar epithelial cells lack enzyme activity which may be inducible through microorganism infection.
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