IMMUNOHISTOCHEMICAL LOCALIZATION OF LAMININ IN FETAL MOUSE LUNGS
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概要
- 論文の詳細を見る
Immunohitochemical localization of laminin was investigated in fetal lungs of mouse. The antigenicitu of laminin was lost during the process of fixation and paraffin embedding, but restored well by the 6O min pepsin trcatment. Laminin was already detected in Day 12 fetal lungs and clearly demarcated the tubule of the lung primordium. In Days 14 and 16 fetal lungs, laminin was found at the epitheliomcsenchymal boundary of any portion of the tubule, and the proximal portion showed stronger inmunoreaction than the distal portion. In Days 17 and 18 fetal lungs, the immunoreaction became generally weak, but the reaction products clearly demarcated the epitheliomesenchymal boundary. In both Day 14 and Day 16 fetal lungs, the effects of the pepsin treatment at different time intervals were explored. Without the pepsin treatment, no peroxidase reaction products were found on any part of the tissue section. When treated with pepsin for 15 min, the immunoreaction became positive at the distal portion of the tubule. After the pepsin treatment for 30 min, the immunoreaction bccame intense at the distal portion and appeared at the proximal portion of the tubulc. The pepsin treatment for 60 min generally intensified the immunoreaction of laminin. However, after the pepsin treatment for 120 min, the immunoreactivity becamc rather weak at the distal portion of the tubule, but was unaltered or became stronger at the proximal portion and at the bronchus. These results indicate that the rapidiy growing or expanding areas in the distal portion of the tubule of fetal lungs appear to be sustained often weakly by the basement membrane which is considered to be immature in its compositions, constituents or matrix assembly, while immunorcactive products of laminin in the basement membrane along the boundary between the undifferentiated epithelium and the adjacent mesenchyme appears consistently in a thick belt-shape.
- 日本組織細胞化学会の論文
著者
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HASHIMOTO Hisashi
Department of Anatomy, Faculty of Medicine, Kyoto University
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HOSHINO Kazumasa
Department of Anatomy, Faculty of Medicine, Kyoto University
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Hashimoto Hisashi
Department Of Anatomy Faculty Of Medicine Kyoto University
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Hashimoto H
Department Of Anatomy Faculty Of Medicine Kyoto University
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Hoshino Kazumasa
Department Of Anatomy Faculties Cf Medicine And Dentistry University Of Manitoba:ludwig-aschoff-haus
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