IDENTIFICATION OF THE MOUSE SUBMANDIBULAR GLAND CELLS IN SITU AND OF ISOLATED SINGLE CELLS WITH A TRIPLE STAINING METHOD

概要

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A newly modified triple stain for DNA with Feulgen reaction using azur B-Schiff reagent, for polysaccharides with PAS reaction using pararosanilin-Schiff reagent, and for proteins with naphthol yellow S facilitates not only cytophotometric study of these three important tissue components utilizing three different wavelengths, but enables identification of at least five different types of submandibular gland cells as well. These cell types include the cells in the acinus, the convoluted secretory tubule, the intercalated duct, and the striated excretory duct; also a "peculiar" cell which is described herein for the first time. Preliminary investigations using cultured L cells revealed that azur B which was once bound to DNA was partially replaced by pararosanilin Schiff reagent, when the latter was subsequently applied. However, when the sequence of the two Schiff reagents was reversed, such a phenomenon was not observed. The color differentiation of the present triple stain was most distinct after glutaraldehyde fixation and least so after acid alcohol fixation. This stain may be applied following embedding in either paraffin, methacrylate, or plastic, and also in isolated single cell preparations. A somewhat improved method of separating single cells from the mouse submandibular gland is also described.
日本組織細胞化学会の論文