THE REACTIONS OF CUPROLINIC BLUE WITH GLYCOCONJUGATE-CONTAINING TISSUES IN LIGHT MICROSCOPY
スポンサーリンク
概要
- 論文の詳細を見る
In a series of mammalian tissues, the reactions of cuprolinic blue with glycoconjugatec were studied under light microscopy. The dye solutions were adjusted to pHs 1.0, 1.5, 2.5, 3.0 and 5.6 and applied to difflerent glycoconjugatc-containing tissues in combination with certain chemical modification and enzyme digestion procedures. The results obtained revealed that (a) the ortho- and metachromatic staining intensities with the dye increase with advancing pH levels, (b) most proteoglycan-containing structures exhibit purple shades of metachromatic stainings at higher pHs, whereas glycoprotein-containing ones show reddish shades of staining at every pH level and (c) the effects of chemical modification and enzyme digestion procedures upon the staining reactions lead to a possible correlation of individual glycoconjugates with their cuprolinic blue stainabilities in tissues.
著者
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Hirabayashi Yoshifumi
Department Molecular Morphology Nagoya City University Graduate School Of Medical Science
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Kyuma Atsushi
Department Of Anatomy Nagoya City University Medical School
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Yamada Kazuyori
Department Of Anatomy Nagoya City University Medical School
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KOMORI SHINGO
Department of Anatomy, Nagoya City University Medical School
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Komori Shingo
Department Of Anatomy Nagoya City University Medical School
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