ELECTRON MICROSCOPIC VISUALIZATION OF ACIDIC GLYCOCONJUGATES BY MEANS OF POSTEMBEDDING PROCEDURES USING RUTHENIUM RED AND TUNGSTATE
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概要
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Postembedding staining methods using ruthenium red (RR) in combination with tungstate (TT) (phospho- or silicotungstic acid) (RR-TT) were tested for the effective visualization of acidic glycoconjugates in electron microscopy. Tissue blocks of trachea and colon from adult male Wistar rats were fixed in phosphate-buffered glutaraldehyde-paraformaldehyde solution and embedded in LR-White. Ultrathin sections were cut, stained first with RR (pH 2.5) and then incubated inTT. In the tissues examined, all the ultrastructures containing acidic glycocon-jugates exhibited apparent positive reaction for RR-TT. If stained singly with RR or TT under the same staining conditions, most of these ultrastructures failed to give any distinct electron densities. In all the acidic glycoconjugate-containing ultrastructures tested, digestion with testicular hyaluronidase greatly diminished or abolished their RR-TT reactions. In view of these results, the present RR-TT methods can be regarded as a useful technique for the efficient visualization of acidic glycoconjugates in electron microscopy.
- 日本組織細胞化学会の論文
著者
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Hirabayashi Yoshifumi
Department Molecular Morphology Nagoya City University Graduate School Of Medical Science
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Yamada Kazuyori
Department Of Anatomy Nagoya City University Medical School
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SAKAGAMI TAKANORI
Department of Anatomy, Nagoya City University Medical School
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Sakagami Takanori
Department Of Anatomy Nagoya City University Medical School
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