ADVANTAGEOUS USAGE OF EELS TO DETECT CA IN PYROANTIMONATE STAINING
スポンサーリンク
概要
- 論文の詳細を見る
Potassium pyroantimonate staining has been utilized to localize calcium (Ca) in biological specimens at the electron microscopic level. This method, however, has been criticized by many investigators because of its specificity to Ca. X-ray microanalysis (EDX) of that reaction product, pyroantimonate-Ca, was an attempt to confirm Ca in it. There is a technical problem in distinguishing Ca and antimony (Sb) by EDX because The characteristic X-ray emmission energies of Sb and Ca are too close each other to be distinguished. Therefore, the reaction product localized in the cytoplasm and over the plasma membrane of type II cells in taste buds of four wild Japanese monkeys was analysed with an electron energy loss spectroscope (EELS) combined with STEM image mode. Ca peak was recognized in the EELS spectrum with the addition of 2% K-pyroantimonate to 4% glutaraldehyde. Similar addition of K-pyroamtimonate to osmium tetroxide was not essential to preserve Ca for EELS survey. Thus, EELS appeared to be more advantageous as compared to EDX in detection of Ca in histochemical reaction product.
- 日本組織細胞化学会の論文
著者
-
Hakoi Kazuo
Department Of Anatomy Kochi Medical School
-
Makita Takashi
Department Of Veterinary Anatomy Faculty Of Agriculture Yamaguchi University
関連論文
- Histology of Vermiform Appendix-Like Organ in Slow Loris
- Histological Study on Intestinal Diverticulum of Tree Shrew (Tupaia Javanica)
- Energy Loss Electron Spectrometric Analysis of the Histochemical Reaction Products II. : Calcium-pyroantimonate.
- Intracellular Localization of Migration Inhibitory Factor-Related Protein (MRP) and Detection of Cell Surface MRP Binding Sites on Human Leukemia Cell Lines^1
- Hepatic histopathology of a vitamin A overdose in mouse liver
- Cytochemical Localization of Ca^-, Mg^-ATPase on the Limiting Membrane of Peroxisomes Isolated from Renal Tubules of Rats
- No effect of voluntary exercise on ovarian follicle in rats
- A Simplified Method of Staining Vitamin A Lipid Droplets in Stellate Cells at the Light and Electron Microscopic Levels
- ホッキョクグマ(Ursus maritimus),ヒグマ(Ursus arctos)およびジャイアントパンダ(Ailuropoda melanoleuca)の橈側種子骨に関する比較解剖学的検討
- Electron Microscopic Enzyme Cytochemistry of the Inductive Effect of Colchicine on the Hepatic Alkaline Phosphatase
- X-ray microanalysis of reaction products of ZIO staining in neurohypophysis of the rat
- BSPT staining of succinate dehydrogenase activity in isolated mitochondria
- A SIMPLE METHOD FOR LIGHT MICROSCOPICAL VISUALIZATION OF PHOSPHOHYDROLASE ACTIVITIES IN MATERIALS PROCESSED FOR CERIUM-BASED ULTRACYTOCHEMISTRY
- Application of clinical test kits to histochemistry. I. Enzymatic determination and staining of total cholesterol in the tissue of rat
- Immunohistochemical localization of swine Cu-Zn-SOD in tissues of swine
- Effect of Swine Cu-Zn-superoxide dismutase (SOD) on ATPase Activity of Gastric Gland
- X-ray Microanalysis of Alkaline Phosphatase Reaction Product on the Lateral Cell Membrane
- ALPase and ATPase activity in three segments of the small intestine of the vitamin D deficient rats
- ADVANTAGEOUS USAGE OF EELS TO DETECT CA IN PYROANTIMONATE STAINING
- Celestin Blue Staining for Glutaraldehyde-fixed Tissues
- Immunocytochemical Localization of Desmin Filaments in Skeletal Muscle of Neonatal Swine
- Immunocytochemical Localization of Desmin in Human Fetal Skeletal Muscle
- Electron Microscopic Immunogold Labeling of Bromodeoxyuridine (BrdU) in Routine Electron Microscopy
- Lectin Histochemistry of the Glandular Part of the Gastric Mucosa of the One Humped Camel (Camelus dromedaries)
- Immunocytochemistry of Apoptosis Induced by Bromodeoxyuridine in Human Leukemic HL-60 Cells
- Double Gallbladder of Swine
- SCANNING ELECTRON MICROSCOPIC OBSERVATIONS OF BIOLOGICAL SECTIONS CUT ON A "VIBRATOME" AND INCUBATED IN MEDIA FOR ATP-ASE AND ALP-ASE ACTIVITY : SEM APPEARANCE OF THE VIBRATOME SECTIONS (SYMPOSIUM ON THE RECENT ADVANCES IN THE ELECTRON MICROSCOPIC CYTOCHE
- Energy Dispersive X-Ray Fluorescence Spectrometry of Major Tissues of Silky Fowls.
- Histological Observation of the Proper Gastric Gland in Minke Whale, Balaenoptera acutorostrata.
- Morphometry of Fine Structural Alterations of Hepatocytes of Japanese Monkeys under Fasting Stress.
- The Surface Color Measurement of Major Tissues of Silky Fowls and White Leghorns.
- Detection of Retinyl Palmitate and Retinol in the Liver of Mice Injected with Excessive Amounts of Retinyl Acetate.