Electron Microscopic Immunogold Labeling of Bromodeoxyuridine (BrdU) in Routine Electron Microscopy
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概要
- 論文の詳細を見る
Incorporated bromodeoxyuridine (BrdU) into DNA was detected in glutaraldehyde and osmium-fixed regenerating rat liver at the electron microscopic level. Without HCl hydrolysis, BrdU was localized in the nuclei of regenerating hepatocytes by post-embedding immunogold technique using the monoclonal anti-BrdU antibody on ultrathin sections after etching of the epoxy resin with 1% sodium ethoxide or 10% H<SUB>2</SUB>O<SUB>2</SUB> followed by trypsin digestion. This procedure localized BrdU in routinely prepared specimens for electron microscopy simultaneously at a much finer structure than ordinary immunoelectron microscopy.
- 日本組織細胞化学会の論文
- 1996-04-01
著者
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Kondo Kazumasa
Department Of Anatomy Kansai Medical University
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Makita T
Department Of Veterinary Anatomy Faculty Of Agriculture Yamaguchi University
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Makita Takashi
Department Of Veterinary Anatomy Faculty Of Agriculture Yamaguchi University
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