ONTOGENY AND LOCALIZATION OF γ-CRYSTALLINS IN THE DEVELOPING RAT LENS : COMPARISON WITH ELEMENTAL ANALYTICAL FINDING BY ENERGY DISPERSION-TYPE ELECTRON PROBE MICROANALYZER
スポンサーリンク
概要
- 論文の詳細を見る
A study of the time of appearance and localization of γ-crystallins was made along with an analysis of component elements in the lens of the developing rat and a correlation between the two was found. The time of appearance and the histological localization of γ-crystallins in developing rat lenses were studied by the immunofluorescent antibody technique. The first positive immunofluorescent reaction with anti-γ-crystallin antibodies was observed at 13 1/2 days in the embryonic lens when cells in the posterior wall of the lens vesicle elongated and differentiated into primary lens fibers as found by Schubert et al. This reaction intensified with the addition of the secondary lens fibers in the lens equator. An elemental analysis of the same samples (used to detect γ-crystallins) using the energy dispersion-type electron probe microanalyzer, revealed that sulphur increased in amount while phosphorus relatively declined in amount with the progress of the formation of the primary and secondary lens fibers. These quantitative changes in sulphur in the X-ray microanalysis are consistent with the appearance and localization of γ-crystallins.
- 日本組織細胞化学会の論文
著者
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Igarashi Hiroaki
2nd Department of Anatomy, Toho University School of Medicine
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Nokubi Kazuto
2nd Dept. Of Anatomy Toho University Of Medicine
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Nokubi Kazuto
2nd Department Of Anatomy Toho Univ. School Of Medicine
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BABA TOSHIKI
Dept. of Ophthalmology, Toho University of Medicine
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KITSUKAWA MAYUMI
Dept. of Ophthalmology, Toho University of Medicine
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Igarashi Hiroaki
2nd Dept. Of Anatomy Toho University Of Medicine
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Igarashi Hiroaki
2nd Department Of Anatomy Toho University School Of Medicine
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Kitsukawa Mayumi
Dept. Of Ophthalmology Toho University Of Medicine
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Baba Toshiki
Dept. Of Ophthalmology Toho University Of Medicine
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- ONTOGENY AND LOCALIZATION OF γ-CRYSTALLINS IN THE DEVELOPING RAT LENS : COMPARISON WITH ELEMENTAL ANALYTICAL FINDING BY ENERGY DISPERSION-TYPE ELECTRON PROBE MICROANALYZER
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