DETECTION OF HISTAMINE IN RAT MAST CELL GRANULES BY ORCEIN-WATER BLUE STAIN

概要

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A new method of staining histamine in rat mast cell granules in formalin fixed paraffin section was developed. Mast cells of several organs of formalin fixed paraffin sections were stained well in red with orcein-water blue as well as in purple with toluidine blue. But only mast cells in dermis showed a different appearance in orcein-water blue stain and toluidine blue stain. The results of an electrophoretic study, diamine oxidase treatment and compound 48/80 treatment of rat lead to the conclusion that the substance stained in red with orcein may be histamine. It was shown that histamine in rat mast cell granule is fixed even by formalin, a finding contrary to previous studies.