A STUDY OF POTASSIUM-OSMIUM-CYANIDE COMPLEX : APPLICATION TO ULTRASTRUCTURAL CYTO-CHEMISTRY WITH DIAMINOBENZIDINE
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概要
- 論文の詳細を見る
Potassium-osmium-cyanide complex (POCC) was substituted for osmium tetroxide procedures for the ultrastructural demonstration of cytochrome oxidase and peroxidase with diaminobenzidine (DAB). POCC reacted with DAB polymer resulting from the oxidation reaction producing electron opaque deposits. POCC produced fine homogeneous staining and more contrast than did the usual osmication method. This suggests a means of making the methods more sensitive. Careful controls are necessary since POCC alone stains smooth membrane elements, thereby possibly obscuring true reaction products in some cases.
- 日本組織細胞化学会の論文
著者
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Seligman Arnold
The Department Of Research Oncology And Cell Biology Fuchu Metropolitan Hospital:the Department Of S
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HOSHINO Yoshinobu
The Department of Urology, Tokyo Cosmoporitan Fuchu Hospital
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SHANNON W.
The Department of Research Oncology and Cell Biology, Fuchu Metropolitan Hospital
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Shannon W.
The Department Of Research Oncology And Cell Biology Fuchu Metropolitan Hospital:the Department Of S
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Hoshino Yoshinobu
The Department Of Research Oncology And Cell Biology Fuchu Metropolitan Hospital:the Department Of S
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