HISTOCHEMICAL DEMONSTRATION OF ACID AND ALKALINE PHOSPHATASE USING 2-NAPHTHYLTHIOLPHOSPHATE AS SUBSTRATE AND OSMIOPHILIC DERIVATIVES AS END PRODUCTS
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概要
- 論文の詳細を見る
Three approaches have been investigated for the histochemical demonstration of acid and alkaline phosphatases with the substrate 2-naphthylthiolphosphate-bisdicyclohexylamine salt, NTP. Each method results in the formation of osmiophilic end-products which are converted to osmium blacks at the sites of acid or alkaline phosphatase activity. Method A gives an osmium black via an intermediate osmiophilic diazothioether formed by capture of enzymatically liberated 2-naphthalenethiol with the diazonium salt, Fast Blue BBN. The other two methods depend upon capture of 2-naphthalenethiol with either ionic cadmium (Method B) or ionic lead (Method C) and subsequent osmication of the cadmium mercaptide or lead mercaptide. In light microscopy, both Methods A and B give better results than Method C for acid phosphatase and especially so for alkaline phosphatase. More enzymatic inhibition of non-lysosomal acid phosphatase is noted with Methods B and C. In electron microscopy, Method A yields deposits in droplet form which are too large to relate properly to underlying ultrastructure. Methods B and C yield deposits that are too crystalline to be useful for relation to fine structure.
- 日本組織細胞化学会の論文
著者
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Ueno Hiromi
The Departments Of Surgery Sinai Hospital Of Baltimore And The Johns Hopkins University School Of Me
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Seligman Arnold
The Department Of Research Oncology And Cell Biology Fuchu Metropolitan Hospital:the Department Of S
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KAWASHIMA TAKAO
The Departments of Surgery, Sinai Hospital of Baltimore and the Johns Hopkins University School of M
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KATZOFF LIONEL
The Departments of Surgery, Sinai Hospital of Baltimore and the Johns Hopkins University School of M
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HANKER JACOB
The Departments of Surgery, Sinai Hospital of Baltimore and the Johns Hopkins University School of M
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Hanker Jacob
The Departments Of Surgery Sinai Hospital Of Baltimore And The Johns Hopkins University School Of Me
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Katzoff Lionel
The Departments Of Surgery Sinai Hospital Of Baltimore And The Johns Hopkins University School Of Me
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Kawashima Takao
The Departments Of Surgery Sinai Hospital Of Baltimore And The Johns Hopkins University School Of Me
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Seligman Arnold
The Departments Of Surgery Sinai Hospital Of Baltimore And The Johns Hopkins University School Of Me
関連論文
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- COMPARATIVE STUDY ON METHODS FOR THE ULTRASTRUCTURAL DEMONSTRATION OF PERIODIC ACID-REACTIVE CARBOHYDRATE MACROMOLECULES
- HISTOCHEMICAL DEMONSTRATION OF ACID AND ALKALINE PHOSPHATASE USING 2-NAPHTHYLTHIOLPHOSPHATE AS SUBSTRATE AND OSMIOPHILIC DERIVATIVES AS END PRODUCTS