HISTOCHEMICAL DEMONSTRATION OF FRUCTOSE 1,6-DIPHOSPHATASE IN RAT LIVER USING NITRO-BLUE TETRAZOLIUM

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概要

• 論文の詳細を見る

• The activity of fructose 1,6-diphosphatase (FDPase) in rat liver tissue was demonstrated histochemically using nitro-blue tetrazolium (nitro-BT) as a hydrogen accepting reagent. A fresh rat liver tissue was frozen by immersing into isopentane kept in an acetone and dry ice mixture. A slice of 8 micron thickness was fixed in a cold acetone (-20℃) for 30 min, covered with a reaction mixture and incubated from 30 to 60 min at 37℃. The incubation mixture is essentially an application of the assay system established for kinetic determination of FDPase activity at neutral pH; the formation of fructose 6-phosphate by FDPase reaction was coupled with a NADPH generating system using phosphoglucose isomerase (PGI) and glucose 6-phosphate dehydrogenase (G-6PDH) reaction. The FDPase activity in liver tissue was demonstrated as a dark blue deposit of formazan in cytoplasm of parenchymal cells and was distributed relatively homogeneously in the lobule. The FDPase activity was inhibited by 5'-AMP, a specific inhibitor of FDPase. The formazan deposit, which was demonstrated in rat liver by the present system, appeared to represent the localization of FDPase activity at neutral pH.

• 日本組織細胞化学会の論文

著者

• Aoe Hajime

  The First Department Of Internal Medicine Okayama University Medical School

関連論文

• HISTOCHEMICAL DEMONSTRATION OF FRUCTOSE 1,6-DIPHOSPHATASE IN RAT LIVER USING NITRO-BLUE TETRAZOLIUM
• Histochemical Demonstration of Human Liver Hexokinase
• HISTOCHEMICAL DEMONSTRATION OF HUMAN LIVER HEXOKINASE USING NITRO-BLUE TETRAZOLIUM
• Histochemical Demonstration of Rat Liver Fructose-1,6-diphosphatase
• HISTOCHEMICAL STUDIES OF HEPATIC HEXOKINASE AND GLUCOSE 6-PHOSPHATASE IN CHRONIC LIVER DISEASES

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