Hyperthermal Influence on Intracellular Free Calcium Concentration in Cultured Macrophages of Mice
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概要
- 論文の詳細を見る
In this study, we have determined the fluorescence value of intracellular free calcium([Ca^<2+>]_i)in macrophages from mice cultured in different temperatures(37℃, 38℃ and 39℃)by laser cytometer. The results indicate that hyperthermia caused elevations in the levels of [Ca^<2+>]_i. The characteristic effects of hyperthermia were, 1)rapid changes in [Ca^<2+>]_i concentration, that is to say, the fluorescence value of [Ca^<2+>]_i rose a few sec after hyperthermia;2)a detectable relationship between the fluorescence value and temperature during defined temperature(38℃-39℃)and time(0-3600 sec), 3)non-synchronous responses of cells, in other words, the extent and time of elevation of [Ca^<2+>]_i fluorescence value were different among the cells of the same group. We thought [Ca^<2+>]_i elevation induced by heat happened through the mechanism of IP^3- induced calcium release(IICR)and calcium-induced calcium release(CICR). The heparin and procaine might lower the concentration of [Ca^<2+>]_i during hyperthermia through the mechanism that IICR and CICR were inhibited respectively. Free calcium influx from the extracellular medium was hot thought to be necessary for heat-induced [Ca^<2+>]_i elevation.
- 日本組織細胞化学会の論文
著者
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Seguchi Harumichi
Department Of Anatomy And Cell Biology Kochi Medical School
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Luo S
Department Of Cell Biology And Medical Genetics First Military Medical University
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Luo Shenqiu
Department Of Anatomy Faculty Of Medicine Kyoto University
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Luo Chen
Department of Cell Biology and Medical Genetics, First Military Medical University
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Yang Cuilan
Department of Cell Biology and Medical Genetics, First Military Medical University
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Piao Yingjie
Department of Cell Biology and Medical Genetics, First Military Medical University
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Luo Chen
Department Of Cell Biology And Medical Genetics First Military Medical University
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Yang Cuilan
Department Of Cell Biology And Medical Genetics First Military Medical University
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Piao Yingjie
Department Of Cell Biology And Medical Genetics First Military Medical University
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