Effects of Extracorporeal Shock Wave Lithotripsy on Na^+-K^+-ATPase Activity in Hepatocyte Plasmalemma of Rabbits

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概要

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• Extracorporeal shock wave lithotripsy (ES-WL) is generally considered as an effective cure for cholelithiasis. The present study is to explore whether ESWL does damage to hepatocytes. After the liver area was exposed to a given period of extracorporeal shock wave (ESW). the livers of 14 rabbits were taken out and samples for electron microscopy were prepared. The Na^+-K^+-ATPase activity in hepatocytes and the extent of damage by ESW was quantified by the cytochemical method using cerium (Ce) as the capture and by the electron probe X-ray microanalysis. It was observed that the Na^+-K^+-ATPase reaction products containing Ce were located on the sinusoidal, lateral and bile canalicular membrane, and were most concentrated on the latter one. The peak and background ratio (P/B) of Ce on the canalicular membrane in the control samples was 1.18±0.36; and in the samples exposed to ESW for 15, 30, 45 min, was 0.88±0.26, 0.86±0.22 and 0.48±0.31 respectively. The differences between the control and ESW-treated samples were statistically significant (p < 0.01). However, the P/B of Ce in the samples of 60^<th> day after 30 min ESW exposure was 1.04±0.26. No difference existed as compared with the control(P > 0.05). In addition, the Ce content of the reaction products on the canalicular membrane decreased to about 1/3 by ouabain treatment. indicating that rich Na^+-K^+-ATPase activity exists. These findings indicate that the ESWL performed on cholelithiasis may exert certain harmful effects on adjacent hepatocytes. Fortunately, the effects disappear within two months.

• 日本組織細胞化学会の論文

著者

• Zhong C

  Dept. Of Histology & Embryology School Of Medicine Fudan University

• ZHONG Cisheng

  Department of Biophysics, Shanghai Medical University

• LING Yiping

  Department of Biophysics, Shanghai Medical University

• Zhong Cisheng

  Faculty Of Basic Medical Sciences Shanghai Medical University

• Shen Xiueheng

  Department of Biophysics, School of Basic Medical Sciences

• Wang Bingsheng

  Department of Surgery, Zhong Shan Hospital, Shanghai Medical University

• Shen X

  Medical Center Of Fudan Univ. Shanghai Chn

• Wang B

  Xian Univ. Medical Univ. Chn

• Ling Yiping

  Department Of Biophysics School Of Basic Medical Sciences

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