Influence of Extracellular Matrix on the Lipogenesis of Cultured White Fat Cells

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Collagenase digests from subcutaneous fattissue of new born rats were cultured on different types of collagen gel containing 50% type l collagen, on fibronectin (Fn) or onlaminin (Lm). On day 6, 17% of cells on aplastic substratum differentiated and hadmultilocular or unilocular cytoplasmic lipiddroplets (CLDs). Cells on each type of col-lagen and on Lm had more CLDs than thoseon a plastic substratum. The extent oflipogenesis showed the following decreasingorder: cells on Lm (80%), on type IV+I collagen (52%), on tvpe III+I collagen (36%), on type II+I collagen (32%). On day 14, most cells on Lm became unilocular fat cells. Cells on Fnshowed delipidation and showed decreasedreactivity to insulin stimulation. Immunohistochemically differentiated fatcells were positively stained with anti-laminin or anti-type IV collagen antibodies.These results provide evidence that Lm andtype IV collagen promote fat cell differentia-tion, whereas Fn facilitates dedifferentiation into the fibroblast-like fat cell.
日本組織細胞化学会の論文