ヒト副腎皮質におけるin vitroのステロイド生合成と代謝

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A study on in vitro bioconversion of steroids in normal and pathological human adrenal glands was performed by isotope tracer method. The materials were from two cases of congenital adrenal hyperplasia, two cases of Cushing's syndrome, one case of prostatic carcinoma which was treated by long-term anti-androgen therapy and one case of essential hypertension not associated with any endocrinological disorders. The last one was used as a normal control. The cell-free homogenate (800xg, supernatant) was prepared from the adrenal gland which was surgicarly removed. The homogenate was incubated with ^<14>C-labeled steroid precursors such as 4-^<14>C-pregnenolone, 4-^<14>C-dehydroepiandrosterone, 4-^<14>C-progesterone, 4-^<14>C-deoxycorticosterone and 4-^<14>C-deoxycortisol. Enzyme reactions were terminated with rapid extraction of steroids with methylene dichloride. Thin-layer chromatography combined with chemical derivative methods was applied for separation and tentative identification of the metabolites. Then, these metabolites were quantitated by measuring the corresponding radioactivitics with a liquid scintillation spectrometer and some of them were identified by constant specific radioactivities through repeated recrystallization with corresponding authentic steroid preparations. Irrespective of whether or not the conditions of in vitro experiments were comparable with the ones observed in vivo, the estimated quantity of the metabolites produced by enzymic reaction in adrenal homogenates could be employed as an index of the related enzymic activity. The results obtained by the present study were as follows.
社団法人日本泌尿器科学会の論文

ヒト副腎皮質におけるin vitroのステロイド生合成と代謝

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