Nude mouse移植と培養細胞系を応用したヒト腎細胞癌の機能と形態に関する研究

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Various clinical manifestations of human renal cell carcinoma have been reported by many authors and it is currently maintained that different functional and morphological characteristics of individual tumor are responsible for this. An attempt to establish cell line has been made for suitable in vitro experimental system. Attempts have also been made to transplant human renal cell carcinoma to nude mouse for further functional and morphological study. Materials and methods : Thirty-six surgical specimens of renal cell carcinoma obtained at Keio University and its affiliated hospitals from September '73 through March '77 were used. Those specimens, after being cultured, transplanted to nude mouse, were examined from different points of view including light and electron microscopic observation, cell growth, chromosomal analysis and determination of erythropoietin activity. Results : 1) Thirtysix cases of direct cell culture were performed in which primary culture and successive culture were successful in 11 and 2 cases, respectively. 2) Six out of 12 transplanted tumors to nude mouse were successful, and 4 of the 6 transplanted tumors were still in growth at present time. Among them included is a tumor capable of producing erythropoietin, which was originally derived from metastatic foci in the lung from renal cell carcinoma. 3) Histopathological findings of the transplanted tumor and original tumor were basically identical, and that of tumors successively cultured also carried the similar characteristics. 4) Indirect culture through nude mouse also succeeded, and among them included was a cell line established and named KU-2 in November 1976. 5) Histopathological characteristics of the established cell line, even after being transplanted back to nude mouse remains unchanged. 6) Presence of brush-border like structure in the transplanted tumor and radially arranged microvilli in cultured cells observed under electron microscopy is a proof for the current thought that renal cell carcinoma is originated in proximal tubular cell. 7) Erythrocytosis transfered to tumor bearing nude mouse as well as determination of erythropoietin activity in the tumor tissue raised the possibility that the tumor per se was capable of producing erythropoietin. Development of hepatomegaly in the nude mouse with this particular tumor, in addition to the above observation, can explain the unique functional characteristics of this tumor.
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