ラット側坐核のドーパミン神経活動への笑気の影響 : in vivo microdialysis による検討

概要

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Using the technique of in vivo microdialysis, extracellular concentration of dopamine, 3, 4-dihydroxypheny acetic acid (DOPAC) and homovanillic acid (HVA) were determined in the nucleus accumbens while Sprague-Dawley rats underwent nitrous oxide inhalation. Microdialysis probe had semipermeable cellulose tubes (molecular weight cutoff 50, 000 : O, D. 0.22mm). Perfusion was started 12 hours after implantation of the microdialysis probe under a freely-moving condition. Mesurement of the extracellular concentration of dopamine, DOPAC and HVA was made at 20 minute intervals, which was started 120 minutes after the onset of the perfusion. Dopamine, DOPAC and HVA were quantified by a high-performance liquid chromatography coupled with an electrochemical detector. Extracellular dopamine levels in the nucleus accumbens increased following nitrous oxide inhalation for 20 minutes. Naloxone does not antagonize the extracellular dopamine levels increasing.
九州歯科学会の論文
1993-02-25