シリアンハムスター頬嚢粘膜癌のリンパ節転移に関する実験的研究

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Cyclosporin A (CsA), an immunosuppressant which inhibits T lymphocytes in an apparently selective manner, and OK-432, an immunoactivator which strengthens the defense mechanism of the host, were tested for their effects on metastasis in a Syrian hamster cheek pouch carcinoma/excision system. The purpose of the present study is to describe the effects of systemically administered immunomodulators on the metastasis of hamster cheek pouch carcinomas after primary tumor excision, and to show that this model has considerable potential for study of the role of immune responses against metastatic tumors. The results obtained were as follows : 1. Lymph node metastasis was found in the left cervical lymph nodes only, the contralateral nodes of all animals showing no metastases. Metastasis to the lung was observed in one animal only, concomitant with lymph node metastasis in the excision with CsA 25 mg group. The incidence of metastasis is summerized as follows : in the excision with CsA 50 mg group, 14 out of 15 animals (93%) showed metastasis. In the excision with CsA 25 mg group, 7 out of 16 animals (44%) showed metastasis. In the excision with OK-432 group, 1 out of 14 animals (7%) showed metastasis. In the excision only group, 6 out of 15 animals (40%) showed metastasis. The difference between the incidence of lymph node metastasis for the excision with CsA 50 mg group and that of the excision only group, as well as for the excision with OK-432 group and that of the excision only group were found to be significant at the p<0.05 level (Fisher's Exact Probability Test), while the incidence for the excision with CsA 25 mg group was similar to that of the excision only group. These results indicate that the incidence of metastasis of this system may be regulated by the immune system of the host, as well as by the tumors themselves. 2. To assess the influence of CsA and OK-432 on the rate of growth of tumors in the lymph node, the metastasis was classified according to five categories by the degree of invasion. The relation between the extent of invasion and the interval after excision may be summarized as follows : in the excision with CsA 50 mg group, 3 animals with stage 1 metastasis were sacrificed between 3 and 5 days after excision, 6 animals with stage 2 were sacrificed between days 7 and 10, and 5 animals with stage 3 were sacrificed between days 17 and 28. In the excision with CsA 25 mg group, 1 animal having stage 1 metastasis was sacrificed on the 6th day, 5 animals with stage 3 were sacrificed between the 15th and the 28th day, and 1 animal with stage 4 was sacrificed on the 26th day. In the excision with OK-432 group, 1 animal with stage 3 was sacrificed on the 28th day. In the excision only group, 6 animals showing metastasis all fell into stage 3 and were all sacrificed on the 28th day after excision. From the above, it appears that both CsA and OK-432 had no effect on the growth of metastasized tumor cells in the lymph node. This data also showed that the arrest and onset of growth of metastatic tumor cells in this system occurs within 6 days after excision.
九州歯科学会の論文
1992-10-25

シリアンハムスター頬嚢粘膜癌のリンパ節転移に関する実験的研究

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