培養マクロファージの Interleukin 1 産生に関する研究
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概要
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Lipopolysaccharide (LPS), Protein A and activated T cells were added to cultures of peritoneal macrophages from rats and the cells were incubated for several hours, and the degree of activation of the macrophages by bacterial antigens (LPS and Protein A) and activated T cells was studied. The degree of the activation was expressed as the proportion of Ia positive cells by count by the fluorescent antibody technique and as the amount of interleukin 1 (IL-1) secreted into the culture medium by ELISA. Results obtained were as follows : 1) The proportion of Ia positive cells and the amount of IL-1 were maximum when LPS and Protein A were added to the cultures at a concentration of 5 μg per ml and the cells were incubated for 6 hr. 2) After LPS addition, the proportion of the cells which expressed Ia antigen during incubation for 1, 2, 3 and 6 hr was 9.5%, 6.3%, 11.6% and 13.8%, respectively. The amount of IL-1 secreted from the cells which were incubated for 1, 2, 3 and 6 hr was 0.57, 0.47, 0.53 and 0.58, respectively. There is a positive correlation between the proportion of Ia positive cells and the amount of IL-1 in the case of the addition of LPS. 3) After the addition of Protein A, the proportion of the cells which expressed Ia antigen during incubation for 1, 2, 3 and 6 hr was 9.2%, 4.5%, 12.7% and 14.9%, respectively. The amount of IL-1 secreted from the cells which were incubated for 1, 2, 3 and 6 hr was 0.75, 0.64, 0.71 and 0.60, respectively. There were no correlations between the proportion of Ia positive cells and the secretion of IL-1. 4) After the addition of activated T cells, the proportion of the cells which expressed Ia antigen during incubation for 1, 2, 3 and 6 hr was 26.0%, 16.7%, 23.0% and 28.0%, respectively. The amount of IL-1 secreted from the cells which were incubated for 1, 2, 3 and 6 hr was 0.69, 0.65, 0.60 and 0.72, respectively. It seemd that the change in the amount of IL-1 was similar to that of the proportion of the cells expressing Ia antigen. 5) The proportion of Ia positive macrophages was 2-3 fold higher in the case of the addition of activated T cells than that in the case of the addition of bacterial antigens. The results that activated T cells cause macrophages in cell culture to activate effectively, probably by the mediation of lymphokins, suggest that the activated T cells will also cause the cells in organs to activate and to modulate immunological response.
- 九州歯科学会の論文
- 1988-12-25